Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.
![All lanes : Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/9525_TCTP-Primary-antibodies-ab133568-2.jpg)
All lanes : Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilutionLane 1 : Raji cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : A431 cell lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique