All lanes : Anti-TFIIE beta antibody [EPR14929] (ab187143) at 1/10000 dilutionLane 1 : K562 lysateLane 2 : Jurkat lysateLane 3 : 293T lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TFIIE beta antibody [EPR14929] (ab187143) at 1/1000 dilutionLane 1 : Mouse heart lysateLane 2 : Rat brain lysateLane 3 : Mouse heart lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunoprecipitation. ab187143 at 1/50 labeling TFIIE beta immunoprecipitated from Jurkat cell lysate using ab187143 at 1/30.
Immunofluorescent analysis of 293T cells labeling TFIIE beta with ab187143 at 1/250 and DAPI staining (blue).
Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling TFIIE beta with ab187143 at 1/250 with a prediluted HRP Polymer for Rabbit IgG as a secondary antibody.
Immunohistochemical analysis of paraffin embedded Human cervix carcinoma tissue labeling TFIIE beta with ab187143 at 1/250 with prediluted HRP Polymer for Rabbit IgG as a secondary antibody.