All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/10000 dilutionLane 1 : rat spleen lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TFPI2 antibody [EPR14442] (ab186747) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Mouse spleen lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling TFPI2 using ab186747 at a 1/250 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Lysate from HeLa cells was immunoprecipitated with ab186747 at a 1/50 dilution. For the subsequent blot, ab186747 was used at a 1/1000 dilution, with a 1/1000 dilution of Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated as the secondary antibody. Blocking/ Dilution buffer: 5% NFDM/TBST.