Anti-TGF beta 3 antibody
| Name | Anti-TGF beta 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab15537 |
| Prices | $398.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P IP |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | A synthetic peptide derived from the C-terminus of the precursor form of human TGF beta 3 |
| Description | Rabbit Polyclonal |
| Gene | TGFB3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.See Abreview
All lanes : Anti-TGF beta 3 antibody (ab15537) at 1/200 dilutionLane 1 : MCF7 (Hu) whole cell lysateLane 2 : U2OS (Hu) whole cell lysateLane 3 : 293T (Hu) whole cell lysateLane 4 : Cos7 (AGMk) whole cell lysateLane 5 : CHO (Hm) whole cell lysateLane 6 : PC12 (Rat) whole cell lysateLane 7 : 3T3 (Ms) whole cell lysateLysates/proteins at 30 µg per lane.SecondaryHRP conjugated swine anti-rabbit antibody at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 50 kDa (why is the actual band size different from the predicted?)Additional bands at : 30 kDa,70 kDa. We are unsure as to the identity of these extra bands.Exposure time : 6 minutesThis image is courtesy of an anonymous AbreviewSee Abreview
ICC/IF image of ab15337 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution + Active human TGF beta 3 full length protein (ab52313) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds
Product References
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Gene transfer and living release of transforming growth factor-beta3 for - Gene transfer and living release of transforming growth factor-beta3 for
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