Anti-TGF beta antibody
| Name | Anti-TGF beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab66043 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ELISA IHC-F ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide (17 aa) derived from C terminal human TGF beta 3 protein |
| Blocking Peptide | Human TGF beta peptide |
| Description | Rabbit Polyclonal |
| Gene | TGFB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human placenta stained with Anti-TGF beta antibody (ab66043) at 1/100.
ab66043 staining TGF beta in Rat liver tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 0.25% serum for 15 minutes at 25°C ; antigen retrieval was by heat mediation in citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/50 in serum free antibody diluent) for 18 hours at 4°C. An undiluted HRP-conjugated Goat polymer was used as the secondary antibody.See Abreview
Anti-TGF beta antibody (ab66043) at 1/500 dilution (for 18 hours at 4°C) + Whole tissue lysate of mouse brain at 20 µgSecondaryAn HRP-conjugated Goat polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Anti-TGF beta antibody (ab66043) at 1/500 dilution + whole tissue lysate prepared from rat intestine at 15 µgSecondaryGoat anti-rabbit conjugated to HRP at 1/5000 dilutiondeveloped using the ECL technique
ICC/IF image of ab66043 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66043, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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