All lanes : Anti-Thioredoxin / TRX antibody [2C9] (ab139677) at 1 µg/mlLane 1 : Thioredoxin fusion protein expressed cell lysate; no induction Lane 2 : Thioredoxin fusion protein expressed cell lysate; induction for 30 minLane 3 : Thioredoxin fusion protein expressed cell lysate; induction for 60 minLane 4 : Thioredoxin fusion protein expressed cell lysate; induction for 90 minLane 5 : Thioredoxin fusion protein expressed cell lysate; induction for 120 minLane 6 : Thioredoxin fusion protein expressed cell lysate; induction for 150 minLane 7 : Thioredoxin fusion protein expressed cell lysate; induction for 180 minLane 8 : Thioredoxin fusion protein expressed cell lysate; induction for 210 mindeveloped using the ECL technique