All lanes : Anti-Thioredoxin domain containing 9 antibody [EPR15238] (ab185959) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : Jurkat cell lysateLane 3 : Raji cell lysateLane 4 : SW480 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Thioredoxin domain containing 9 with ab185959 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab185959 at 1/50 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunohistochemical analysis of paraffin-embedded Human astocytoma tissue labeling Thioredoxin domain containing 9 with ab185959 at 1/50 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
All lanes : Anti-Thioredoxin domain containing 9 antibody [EPR15238] (ab185959) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution