Anti-Thymidine Kinase 1 antibody [3B3.E11]
| Name | Anti-Thymidine Kinase 1 antibody [3B3.E11] |
|---|---|
| Supplier | Abcam |
| Catalog | ab988 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3B3.E11 |
| Applications | FC ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Amino terminal-His-tagged recombinant human thymidine kinase |
| Description | Mouse Monoclonal |
| Gene | TK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab988 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab988, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-Thymidine Kinase 1 antibody [3B3.E11] (ab988) at 1/500 dilution + Whole cell lysate prepared from human SW620 cells at 25 µgSecondaryHRP conjugated pig dakocytomation at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 32 kDa (why is the actual band size different from the predicted?)Additional bands at : 38 kDa (possible non-specific binding).Exposure time : 5 minutesThis image is courtesy of an anonymous AbreviewSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10773_ab988-11846_copy.jpg)
Anti-Thymidine Kinase 1 antibody [3B3.E11] (ab988) at 1/500 dilution + Whole cell lysate prepared from human SW620 cells at 25 µgSecondaryHRP conjugated pig dakocytomation at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 32 kDa (why is the actual band size different from the predicted?)Additional bands at : 38 kDa (possible non-specific binding).Exposure time : 5 minutesThis image is courtesy of an anonymous AbreviewSee Abreview
![Overlay histogram showing HeLa cells stained with ab988 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab988, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/10774_Thymidine-Kinase-1-Primary-antibodies-ab988-1.jpg)
Overlay histogram showing HeLa cells stained with ab988 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab988, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo - Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo
Chan F, Sun C, Perumal M, Nguyen QD, Bavetsias V, McDonald E, Martins V, Wilsher NE, Raynaud FI, Valenti M, Eccles S, Te Poele R, Workman P, Aboagye EO, Linardopoulos S. Mol Cancer Ther. 2007 Dec;6(12 Pt 1):3147-57.
IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 - IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1
Tu Z, Prajapati S, Park KJ, Kelly NJ, Yamamoto Y, Gaynor RB. J Biol Chem. 2006 Mar 10;281(10):6699-706. Epub 2006 Jan 5.
Fluorodeoxyuridine modulates cellular expression of the DNA base excision repair - Fluorodeoxyuridine modulates cellular expression of the DNA base excision repair
Fischer JA, Muller-Weeks S, Caradonna SJ. Cancer Res. 2006 Sep 1;66(17):8829-37.
Isolation and characterization of Dictyostelium thymidine kinase 1 as a - Isolation and characterization of Dictyostelium thymidine kinase 1 as a
O'Day DH, Chatterjee-Chakraborty M, Wagler S, Myre MA. Biochem Biophys Res Commun. 2005 Jun 17;331(4):1494-502.