Anti-Thymosin beta 10 antibody

• Supplier: Abcam
• Catalog: ab14338
• Prices: $370.00
• Sizes: 50 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide: KRSEIS conjugated to KLH, corresponding to amino acids 38-43 of Human Thymosin beta 10
• Description: Rabbit Polyclonal
• Gene: TMSB10
• Conjugate: Unconjugated

Product images

ICC/IF image of ab14338 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14338, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14338 at 1/1000 dilution.

Product References

Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution. - Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution.

Lagarrigue M, Becker M, Lavigne R, Deininger SO, Walch A, Aubry F, Suckau D, Pineau C. Mol Cell Proteomics. 2011 Mar;10(3):M110.005991.

Overexpression of thymosin beta-10 inhibits VEGF mRNA expression, autocrine VEGF - Overexpression of thymosin beta-10 inhibits VEGF mRNA expression, autocrine VEGF

Zhang T, Li X, Yu W, Yan Z, Zou H, He X. Ophthalmic Res. 2009;41(1):36-43.