Anti-Thymosin beta 4 antibody
| Name | Anti-Thymosin beta 4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab14335 |
| Prices | $390.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rat, Chicken, Human |
| Antigen | Synthetic peptide: SDKPDMAEIEK conjugated to KLH, corresponding to amino acids 1-11 of Human Thymosin beta 4 |
| Description | Rabbit Polyclonal |
| Gene | TMSB4X |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab14335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14335, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of adult rat testis tissue, staining Thymosin beta 10 with ab14335 at 1/1000 dilution.