ab87764 staining Thyrotropin Releasing Hormone (TRH) in chicken brain sections by Immunohistochemistry (Frozen sections).The tissue was fixed in paraformaldehyde, permeabilized using PBS/ 0.1% Triton X-100, blocked with 4% BSA, 5% normal goat serum, 0.1M lysine and 0.1% Triton X-100 in PBS and then incubated with ab87764 at a 1/100 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.Antigen retrieval was performed with a Tris-EDTA buffer, pH 9 (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween 20, pH 9.0).See Abreview