Lane 1 : Anti-TIM 1 antibody (ab47635) at 1 µg/mlLane 2 : Anti-TIM 1 antibody (ab47635) at 2 µg/mlLane 1 : Human Uterus Tissue LysateLane 2 : Human Uterus Tissue LysateLysates/proteins at 15 µg per lane.
ab47635 at 10 ug/ml staining TIM1 in human uterus tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
ICC/IF image of ab47635 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47635, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TIM-1 in Human Uterus cells using ab47635 at 20 ug/ml.