All lanes : Anti-TIM 1 antibody [EPR6383(2)] - N-terminal (ab190696) at 1/50000 dilutionLane 1 : 293 cell lysateLane 2 : HeLa cell lysateLane 3 : NCCIT cell lysateLane 4 : IM-9 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TIM 1 antibody [EPR6383(2)] - N-terminal (ab190696) at 1/10000 dilutionLane 1 : C6 cell lysateLane 2 : Raw264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TIM 1 antibody [EPR6383(2)] - N-terminal (ab190696) at 1/1000 dilution + Human TIM 1 recombinant protein at 0.01 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution