Anti-TIMM17A antibody [EPR16098]

• Supplier: Abcam
• Catalog: ab192246
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16098
• Applications: ICC/IF ICC/IF IP WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TIMM17A aa 150 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: TIMM17A
• Conjugate: Unconjugated

Product images

All lanes : Anti-TIMM17A antibody [EPR16098] (ab192246) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-TIMM17A antibody [EPR16098] (ab192246) at 1/1000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : PC-12 cell lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling TIMM17A with ab192246 at 1/50 dilution (11.7 μg/ml), followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are ab192246 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

Western blot analysis of TIMM17A in HeLa cell lysates immunoprecipitated using ab192246 at 1/40 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/Dilution buffer: 5% NFDM /TBST.