Anti-TIMM44 antibody [EPR16821]

• Supplier: Abcam
• Catalog: ab194829
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16821
• Applications: WB ICC/IF ICC/IF IP IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Recombinant fragment within Human TIMM44 aa 1-250
• Description: Rabbit Monoclonal
• Gene: TIMM44
• Conjugate: Unconjugated

Product images

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIMM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed.

Anti-TIMM44 antibody [EPR16821] (ab194829) at 1/1000 dilution + mouse heart lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of A431 cells labeling TIMM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on A431 cell line was observed.

All lanes : Anti-TIMM44 antibody [EPR16821] (ab194829) at 1/5000 dilutionLane 1 : A431 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1).  Lane 2: PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.