Anti-TIMP2 antibody [3A4]

Name	Anti-TIMP2 antibody [3A4]
Supplier	Abcam
Catalog	ab1828
Prices	$400.00
Sizes	250 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone	3A4
Applications	ICC/IF ICC/IF WB IHC-P IHC-F
Species Reactivities	Mouse, Rat, Rabbit, Guinea Pig, Bovine, Human
Antigen	BALB/C mice were injected with a synthetic peptide from the N-terminal region of human TIMP 2
Description	Mouse Monoclonal
Gene	TIMP2
Conjugate	Unconjugated
Supplier Page	Shop

Product images

ICC/IF image of ab1828 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1828, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TIMP2 antibody [3A4] (ab109708) at 5 µg/ml + Recombinant TIMP2 at 0.1 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 24 kDa (why is the actual band size different from the predicted?)Exposure time : 20 minutes

IHC image of TIMP2 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1828, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

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