ab62251 at 1/50 - 1/100 dilution staining Tissue Factor in Human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Anti-Tissue Factor (phospho S290) antibody (ab62251) at 1/2000 dilution + whole cell lysate prepared from transfected human endothelial cells expressing the human full length Tissue Factor (phospho S290) (treated with PAR2-agonist peptide for 90 minutes) at 20 µg/mlSecondaryGoat anti-rabbit IgG-HRP at 1/1000 dilutionObserved band size : 46 kDa (why is the actual band size different from the predicted?)Image courtesy of Dr C Ettelaie by Abreview.See Abreview