Lane 1 : Anti-TLR1 antibody (ab37068) at 1 µg/mlLane 2 : Anti-TLR1 antibody (ab37068) at 2 µg/mlLane 3 : Anti-TLR1 antibody (ab37068) at 4 µg/mlLane 1 : Mouse spleen lysateLane 2 : Mouse spleen lysateLane 3 : Mouse spleen lysateLysates/proteins at 15 µg per lane.
ab37068 at 10µg/ml staining TLR1 in mouse spleen tissue by IHC
ICC/IF image of ab37068 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TLR1 in Mouse Spleen cells using ab37068 at 20 ug/ml.