All lanes : Anti-TLR1 antibody (ab22057) at 2 µg/mlLane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab3955)Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate (ab7187)Observed band size : 87 kDa (why is the actual band size different from the predicted?)Additional bands at : 190 kDa,32 kDa,60 kDa. We are unsure as to the identity of these extra bands.
ab22057, at 4ug/106, staining TLR1 in permeabilised Ramos cells by flow ctometric analysis (open histogram).