Anti-TLR4 antibody [HTA125]
| Name | Anti-TLR4 antibody [HTA125] |
|---|---|
| Supplier | Abcam |
| Catalog | ab30667 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | HTA125 |
| Applications | WB FC IP B/N ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Monkey |
| Antigen | Ba/F3 cell line expressing TLR4 |
| Description | Mouse Monoclonal |
| Gene | TLR4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab30667 staining TLR4 in human peripheral blood monocytes by Flow Cytometry analysis.
ICC/IF image of ab30667 stained rat adrenal medulla PC12 cells. The cells were 4% PFA and 100% Methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30667, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
Product References
Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB - Lipopolysaccharide activates Toll-like receptor 4 (TLR4)-mediated NF-kappaB
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Modified low density lipoprotein stimulates complement C3 expression and - Modified low density lipoprotein stimulates complement C3 expression and
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HMGB1 release and redox regulates autophagy and apoptosis in cancer cells. - HMGB1 release and redox regulates autophagy and apoptosis in cancer cells.
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Baculovirus transduction of mesenchymal stem cells triggers the toll-like - Baculovirus transduction of mesenchymal stem cells triggers the toll-like
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Toll-like receptors on human mesenchymal stem cells drive their migration and - Toll-like receptors on human mesenchymal stem cells drive their migration and
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TNF-alpha induction by Pseudomonas aeruginosa lipopolysaccharide or - TNF-alpha induction by Pseudomonas aeruginosa lipopolysaccharide or
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Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting - Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting
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Signal transduction and nuclear responses in Staphylococcus aureus-induced - Signal transduction and nuclear responses in Staphylococcus aureus-induced
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