Anti-TM4SF3 antibody
| Name | Anti-TM4SF3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab70007 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide derived from an internal region of human TM4SF3 |
| Description | Rabbit Polyclonal |
| Gene | TSPAN8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TM4SF3 antibody (ab70007) at 1/500 dilutionLane 1 : 293 cell extractLane 2 : COLO cell extractLane 3 : COLO cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
Immunofluorescence of HeLa cells treated with ab70007 at 1/500 dilution. Right panel was treated with immunising peptide.
IHC image of ab70007 staining in human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.