All lanes : Anti-TMEM85 antibody [EPR15080] (ab184544) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : U87-MG cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TMEM85 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TMEM85 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TMEM85 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.
Western blot analysis of TMEM85 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2: PBS.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.