All lanes : Anti-TMEM85 antibody [EPR15081] (ab184162) at 1/5000 dilutionLane 1 : 293 cell lysateLane 2 : SH-SY5Y cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
All lanes : Anti-TMEM85 antibody [EPR15081] (ab184162) at 1/20000 dilutionLane 1 : U87-MG cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TMEM85 with ab184162 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.