All lanes : Anti-TMPRSS11A antibody - SEA Domain (ab59846) at 1/1000 dilutionLane 1 : Tissue lysate prepared from mouse embryo fibroblast C3H/10T cells (1:2) (treated with SF) at 30 µlLane 2 : Tissue lysate prepared from mouse embryo fibroblast C3H/10T cells (1:2) (treated with RA) at 30 µlLane 3 : Tissue lysate prepared from mouse embryo fibroblast C3H/10T cells (1:2) (treated with SF) at 20 µlLane 4 : Tissue lysate prepared from mouse embryo fibroblast C3H/10T cells (1:2) (treated with RA) at 20 µl