All lanes : Anti-TMPRSS13 antibody (ab59863) at 1/1000 dilutionLane 1 : Cell media from human colon SW620 cells (treated with SF) at 20 µlLane 2 : Cell media from human colon SW620 cells (treated with TPA) at 20 µlLane 3 : Cell media from human colon SW620 cells (treated with IL1-beta) at 20 µlLane 4 : Cell media from human colon SW620 cells (treated with TNF-alpha) at 20 µlLane 5 : Cell media from human colon SW620 cells (treated with RA) at 20 µlLane 6 : Cell media from human amnion-derived WISH cells (treated with IL1-beta) at 35 µl