All lanes : Anti-TMUB2 antibody [EPR13741] (ab181183) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HEK293 cell lysateLane 3 : HepG2 cell lysateLane 4 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometry analysis of TMUB2 expression in HeLa cells using ab181183 at 1/150 dilution (red) and a rabbit IgG as negative control (green).
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or negative control (lane 2), labeling TMUB2 immunoprecipitated using ab181183 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.