Anti-TNF alpha antibody
| Name | Anti-TNF alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9739 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | unknown |
| Applications | IHC-F IHC-P WB ELISA B/N ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Highly pure (>98%) recombinant mTNF-alpha (mouse Tumor Necrosis Factor-alpha) |
| Description | Rabbit Polyclonal |
| Gene | TNF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TNF alpha antibody (ab9739) at 1/3500 dilutionLane 1 : 100ug LPS stimulated J774.A1 mice macrophagesLane 2 : 50ug LPS stimulated J774.A1 mice macrophagesLane 3 : 25ug LPS stimulated J774.A1 mice macrophagesSecondaryHRP conjugated goat anti-rabbit antibodyPerformed under reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)This image is courtesy of an Abreview submitted by Dr sandra sobocanecSee Abreview
ab9739 (2µg/ml) staining TNF alpha in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining within the germinal follicle.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab9739 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9739 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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