Anti-TNF alpha antibody
| Name | Anti-TNF alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9635 |
| Prices | $395.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA B/N ELISA |
| Species Reactivities | Human |
| Antigen | Highly pure (>98%) recombinant hTNF-alpha (human Tumor Necrosis Factor-alpha); catalogue number ab9642 |
| Description | Rabbit Polyclonal |
| Gene | TNF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9635, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Standard curve for TNF alpha (Analyte: ab9642); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2C8] to TNF alpha (ab8348) at 5µg/ml and Detector Antibody Rabbit polyclonal to TNF alpha (ab9635) at 0.5µg/ml.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12694_TNF-alpha-Primary-antibodies-ab9635-10.jpg)
Standard curve for TNF alpha (Analyte: ab9642); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [2C8] to TNF alpha (ab8348) at 5µg/ml and Detector Antibody Rabbit polyclonal to TNF alpha (ab9635) at 0.5µg/ml.
developed using the ECL techniquePerformed under reducing conditions.
Anti-TNF alpha antibody (ab9635) at 0.2 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 26 kDa (why is the actual band size different from the predicted?)Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
Product References
Cathepsin B contributes to autophagy-related 7 (Atg7)-induced nod-like receptor 3 - Cathepsin B contributes to autophagy-related 7 (Atg7)-induced nod-like receptor 3
Li S, Du L, Zhang L, Hu Y, Xia W, Wu J, Zhu J, Chen L, Zhu F, Li C, Yang S. J Biol Chem. 2013 Oct 18;288(42):30094-104.
Multiplexed protein analysis using encoded antibody-conjugated microbeads. - Multiplexed protein analysis using encoded antibody-conjugated microbeads.
Theilacker N, Roller EE, Barbee KD, Franzreb M, Huang X. J R Soc Interface. 2011 Aug 7;8(61):1104-13.