Anti-TNF alpha antibody (ab183896) at 1/1000 dilution + Raji whole cell lysate/extract at 30 µg
All lanes : Anti-TNF alpha antibody (ab183896) at 1/5000 dilutionLane 1 : 293T whole cell lysate/extractLane 2 : TNF alpha-transfected 293T whole cell lysate/extractLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma, labeling TNF alpha using ab183896 at a 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, labeling TNF alpha using ab183896 at a 1/500 dilution.
Immunofluorescence analysis of Jurkat cells fixed in 4% paraformaldehyde at RT for 15 minutes, labeling TNF alpha using ab183896 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.