All lanes : Anti-TNF Receptor II antibody (ab74315) at 1 µg/mlLane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate (ab76828)Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutionPerformed under reducing conditions.
All lanes : Anti-TNF Receptor II antibody (ab74315) at 1 µg/mlLane 1 : Heart (Rat) Tissue LysateLane 2 : Thymus (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.