Anti-TNFAIP3 antibody [59A426]
| Name | Anti-TNFAIP3 antibody [59A426] |
|---|---|
| Supplier | Abcam |
| Catalog | ab13597 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 59A426 |
| Applications | IHC-P FC WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fusion protein of full length TNFAIP3 (Human) |
| Description | Mouse Monoclonal |
| Gene | TNFAIP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue labelling TNFAIP3 with ab13597 at 5µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
![Anti-TNFAIP3 antibody [59A426] (ab13597) at 4 µg/ml + Jurkat cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12793_ab13597-206522-ab13597wb.jpg)
Anti-TNFAIP3 antibody [59A426] (ab13597) at 4 µg/ml + Jurkat cell lysate
![All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 90 kDa (why is the actual band size different from the predicted?)Additional bands at : 15 kDa,34 kDa. We are unsure as to the identity of these extra bands.Exposure time : 20 minutes](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12794_TNFAIP3-Primary-antibodies-ab13597-4.jpg)
All lanes : Anti-TNFAIP3 antibody [59A426] (ab13597) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 90 kDa (why is the actual band size different from the predicted?)Additional bands at : 15 kDa,34 kDa. We are unsure as to the identity of these extra bands.Exposure time : 20 minutes
![Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/12795_TNFAIP3-Primary-antibodies-ab13597-5.jpg)
Overlay histogram showing HepG2 cells stained with ab13597 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13597, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HepG2 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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