Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121716 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ab121716 at 1/250 dilution staining TOM1L2 in Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
All lanes : Anti-TOM1L2 antibody (ab121716) at 1/250 dilutionLane 1 : RT4 cell lysateLane 2 : U251 MG cell lysateLane 3 : Human PlasmaLane 4 : Human Liver tissue lysateLane 5 : Human Tonsil tissue lysatedeveloped using the ECL technique
Immunohistochemical staining of Human heart muscle shows moderate cytoplasmic positivity in myocytes using ab121716