Anti-TOMM20 antibody

• Supplier: Abcam
• Catalog: ab56783
• Prices: $404.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Applications: WB IHC-P FC IP ICC/IF ICC/IF
• Species Reactivities: Rat, Human, Mouse
• Antigen: Recombinant full length protein, corresponding to amino acids 1-146 of Human TOMM20
• Description: Mouse Monoclonal
• Gene: TOMM20
• Conjugate: Unconjugated

Product images

TOMM20 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to TOMM20 (ab56783) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56783.Secondary: Protein G-HRP at 1/500 dilution.Band: 14kDa: TOMM20. Non specific - 25kDa: We are unsure as to the identity of this extra band.

TOMM20 antibody (ab56783) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.

ICC/IF image of rat primary Hippocampal culture stained with ab56783. The cells on cover slip were incubated in 10% donkey serum in 0.1% PBS- and 0.3x triton1000 for 1h to permeabilise the cells to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56783, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.See Abreview

Overlay histogram showing HeLa cells stained with ab56783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56783, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.

Product References

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