![Overlay histogram showing HeLa cells stained with ab115746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115746, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13056_ab115746-7-ab115746FC.jpg)
Overlay histogram showing HeLa cells stained with ab115746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115746, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab115746 at 10ug/ml staining TOMM20 in Human kidney by immunohistochemistry (FFPE).
![Anti-TOMM20 antibody [4F3] (ab115746) at 1/500 dilution + HeLa cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13058_TOMM20-Primary-antibodies-ab115746-2.jpg)
Anti-TOMM20 antibody [4F3] (ab115746) at 1/500 dilution + HeLa cell lysate
![Anti-TOMM20 antibody [4F3] (ab115746) at 1/500 dilution + PC12 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13059_TOMM20-Primary-antibodies-ab115746-3.jpg)
Anti-TOMM20 antibody [4F3] (ab115746) at 1/500 dilution + PC12 cell lysate
![Anti-TOMM20 antibody [4F3] (ab115746) at 1/500 dilution + NIH3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13060_TOMM20-Primary-antibodies-ab115746-4.jpg)
Anti-TOMM20 antibody [4F3] (ab115746) at 1/500 dilution + NIH3T3 cell lysate
ab115746 at 10ug/ml staining TOMM20 in HeLa cells by immunofluorescence.
ab115746 binding to recombinant TOMM20 by ELISA. Detection limit for recombinant tagged TOMM20 is approximately 0.1 ng/ml as a capture antibody.