Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

• Supplier: Abcam
• Catalog: ab186735
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15581-54
• Applications: ICC/IF ICC/IF WB IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human TOMM20 aa 1 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: TOMM20
• Conjugate: Unconjugated

Product images

All lanes : Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : SH-SY5Y cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735) at 1/2000 dilutionLane 1 : PC-12 cell lysateLane 2 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling TOMM20 with ab186735 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TOMM20 (green) with ab186735 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

Flow cytometry analysis of TOMM20 expression in paraformaldehyde-fixed HeLa cells cells using ab186735 at 1/90 dilution (red) and a rabbit IgG as negative control (green).