Anti-TOMM20 antibody - Mitochondrial Marker

• Supplier: Abcam
• Catalog: ab78547
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IP WB IHC-P
• Species Reactivities: Human, Mouse, Rat, Orangutan
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human TOMM20
• Description: Rabbit Polyclonal
• Gene: TOMM20
• Conjugate: Unconjugated

Product images

ICC/IF image of ab78547 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78547, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HepG2, HeLa, Hek293, MCF-7 cells at 1µg/ml.

Anti-TOMM20 antibody - Mitochondrial Marker (ab78547) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of TOMM20 staining in Human Liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78547, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

TOMM20 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to TOMM20 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab78547.Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.Band: 16kDa; TOMM20.

Product References

Hepatitis C virus induces the mitochondrial translocation of Parkin and - Hepatitis C virus induces the mitochondrial translocation of Parkin and

Kim SJ, Syed GH, Siddiqui A. PLoS Pathog. 2013 Mar;9(3):e1003285.

Hepatitis B virus disrupts mitochondrial dynamics: induces fission and mitophagy - Hepatitis B virus disrupts mitochondrial dynamics: induces fission and mitophagy

Kim SJ, Khan M, Quan J, Till A, Subramani S, Siddiqui A. PLoS Pathog. 2013;9(12):e1003722.

The conserved GTPase Gem1 regulates endoplasmic reticulum-mitochondria - The conserved GTPase Gem1 regulates endoplasmic reticulum-mitochondria

Kornmann B, Osman C, Walter P. Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14151-6. doi: