Phospho-α-E-Catenin (Ser655/Thr658) Antibody

• Supplier: Cell Signaling Technology
• Catalog: 13231
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB IP
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser655/Thr658 of human α-E-catenin protein
• Description: Rabbit Polyclonal
• Gene: CTNNA1

Product images

Western blot analysis of extracts from various cell lines using Phospho-α-E-Catenin (Ser655/Thr658) Antibody.

Western blot analysis of extracts from NCI-H3255 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-α-E-Catenin (Ser655/Thr658) Antibody (upper), α-E-Catenin (23B2) Rabbit mAb #3240 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Phospho-α-E-Catenin (Ser655/Thr658) Antibody specificity was determined by peptide competition ELISA. The binding of Phospho-α-E-Catenin (Ser655/Thr658) Antibody to phospho-α-E-catenin (Ser655/Thr658) peptide in the presence of increasing concentrations of competitor peptides is shown. Only the di-phospho-α-E-catenin (Ser655/Thr658) peptide competes for binding.

Immunoprecipitation of phospho-α-E-catenin protein from PANC-1 cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-α-E-Catenin (Ser655/Thr658) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-α-E-Catenin (Ser655/Thr658) Antibody.