Anti-Torsin A antibody
| Name | Anti-Torsin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab34540 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Rat Torsin A |
| Description | Rabbit Polyclonal |
| Gene | TOR1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Torsin A antibody (ab34540) at 1 µg/mlLane 1 : MarkerLane 2 : Mouse Brain at 10 µgLane 3 : Testis (Mouse) Whole Cell Lysate - normal tissue at 10 µgLane 4 : Mouse Heart Mouse Tissue Lysate at 10 µgLane 5 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711) at 10 µgLane 6 : Mouse Fetal lysate - 14 Days old Tissue Lysate at 10 µgLane 7 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µgLane 8 : Mouse Embryonic Fibroblast 1 at 10 µgLane 9 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179) at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
All lanes : Anti-Torsin A antibody (ab34540) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 3 : Liver (Mouse) Tissue Lysate - normal tissueLane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 5 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilutionPerformed under reducing conditions.
ICC/IF image of ab34540 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34540, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
IHC-FoFr image of Torsin A (ab34540) on Rat Cerebral Cortex section. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.See Abreview
Product References
TorsinA hypofunction causes abnormal twisting movements and sensorimotor circuit - TorsinA hypofunction causes abnormal twisting movements and sensorimotor circuit
Liang CC, Tanabe LM, Jou S, Chi F, Dauer WT. J Clin Invest. 2014 Jul;124(7):3080-92.
Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry. - Torsin A Localization in the Mouse Cerebellar Synaptic Circuitry.
Puglisi F, Vanni V, Ponterio G, Tassone A, Sciamanna G, Bonsi P, Pisani A, Mandolesi G. PLoS One. 2013 Jun 19;8(6):e68063. Print 2013.
Pre-synaptic release deficits in a DYT1 dystonia mouse model. - Pre-synaptic release deficits in a DYT1 dystonia mouse model.
Yokoi F, Cheetham CC, Campbell SL, Sweatt JD, Li Y. PLoS One. 2013 Aug 13;8(8):e72491.
The ubiquitin ligase F-box/G-domain protein 1 promotes the degradation of the - The ubiquitin ligase F-box/G-domain protein 1 promotes the degradation of the
Gordon KL, Glenn KA, Bode N, Wen HM, Paulson HL, Gonzalez-Alegre P. Neuroscience. 2012 Nov 8;224:160-71.
Developmental profile of the aberrant dopamine D2 receptor response in striatal - Developmental profile of the aberrant dopamine D2 receptor response in striatal
Sciamanna G, Tassone A, Martella G, Mandolesi G, Puglisi F, Cuomo D, Madeo G, Ponterio G, Standaert DG, Bonsi P, Pisani A. PLoS One. 2011;6(9):e24261.
A molecular mechanism underlying the neural-specific defect in torsinA mutant - A molecular mechanism underlying the neural-specific defect in torsinA mutant
Kim CE, Perez A, Perkins G, Ellisman MH, Dauer WT. Proc Natl Acad Sci U S A. 2010 May 25;107(21):9861-6. doi:
Chemical enhancement of torsinA function in cell and animal models of torsion - Chemical enhancement of torsinA function in cell and animal models of torsion
Cao S, Hewett JW, Yokoi F, Lu J, Buckley AC, Burdette AJ, Chen P, Nery FC, Li Y, Breakefield XO, Caldwell GA, Caldwell KA. Dis Model Mech. 2010 May-Jun;3(5-6):386-96.
Earlier onset of motor deficits in mice with double mutations in Dyt1 and Sgce. - Earlier onset of motor deficits in mice with double mutations in Dyt1 and Sgce.
Yokoi F, Yang G, Li J, DeAndrade MP, Zhou T, Li Y. J Biochem. 2010 Oct;148(4):459-66.
Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism - Dopamine D2 receptor dysfunction is rescued by adenosine A2A receptor antagonism
Napolitano F, Pasqualetti M, Usiello A, Santini E, Pacini G, Sciamanna G, Errico F, Tassone A, Di Dato V, Martella G, Cuomo D, Fisone G, Bernardi G, Mandolesi G, Mercuri NB, Standaert DG, Pisani A. Neurobiol Dis. 2010 Jun;38(3):434-45.
The dystonia-associated protein torsinA modulates synaptic vesicle recycling. - The dystonia-associated protein torsinA modulates synaptic vesicle recycling.
Granata A, Watson R, Collinson LM, Schiavo G, Warner TT. J Biol Chem. 2008 Mar 21;283(12):7568-79.