Anti-TPD52 antibody (ab77459) at 5 µg/ml + immunogen at 0.2 µgSecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
All lanes : Anti-TPD52 antibody (ab77459) at 5 µg/mlLane 1 : TPD52 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Detection limit for this product is ~1ng/ml as a capture antibody.
IHC image of ab77459 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77459, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.