Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/10000 dilution + Mouse testis lysate at 20 µgSecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
All lanes : Anti-TPGS1 antibody [EPR14324] (ab184178) at 1/2000 dilutionLane 1 : Rat testis lysateLane 2 : SH-SY5Y lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Immunoprecipitation analysis of SH-SY5Y cells labeling TPGS1 using ab184178 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
Flow cytometry analysis of 2% paraformaldehyde fixed SH-SY5Y cells staining TPGS1 using ab184178 at 1/50 and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve), and a Rabbit monoclonal IgG negative control (green curve).