ab111828 staining TPH2 in Mouse dorsal raphe coronal brain slices tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 2% serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/800 in PBS + Triton X-100 + serum) at 22°C for 24 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/500 in PBS + Goat serum) was used as the secondary antibody.See Abreview