Anti-TRA-1-81 antibody [TRA-1-81]
| Name | Anti-TRA-1-81 antibody [TRA-1-81] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16289 |
| Prices | $403.00 |
| Sizes | 200 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | TRA-1-81 |
| Applications | ICC/IF ICC/IF FC WB IP ICC/IF RIA IHC-F |
| Species Reactivities | Rabbit, Human |
| Antigen | Human embryonal carcinoma cell line 2102Ep cl |
| Description | Mouse Monoclonal |
| Gene | PODXL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µgSecondaryGoat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilutiondeveloped using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/13602_ab16289%25203%2520mins%2520copy.jpg)
Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µgSecondaryGoat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilutiondeveloped using the ECL technique
ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.See Abreview
ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.See Abreview
Product References
Derivation of novel human ground state naive pluripotent stem cells. - Derivation of novel human ground state naive pluripotent stem cells.
Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D, Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, Novershtern N, Hanna JH. Nature. 2013 Dec 12;504(7479):282-6.
Amniocytes can serve a dual function as a source of iPS cells and feeder layers. - Amniocytes can serve a dual function as a source of iPS cells and feeder layers.
Anchan RM, Quaas P, Gerami-Naini B, Bartake H, Griffin A, Zhou Y, Day D, Eaton JL, George LL, Naber C, Turbe-Doan A, Park PJ, Hornstein MD, Maas RL. Hum Mol Genet. 2011 Mar 1;20(5):962-74.
Utilization of human amniotic mesenchymal cells as feeder layers to sustain - Utilization of human amniotic mesenchymal cells as feeder layers to sustain
Zhang K, Cai Z, Li Y, Shu J, Pan L, Wan F, Li H, Huang X, He C, Liu Y, Cui X, Xu Y, Gao Y, Wu L, Cao S, Li L. Cell Reprogram. 2011 Aug;13(4):281-8.
A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells - A virus-free poly-promoter vector induces pluripotency in quiescent bovine cells
Huang B, Li T, Alonso-Gonzalez L, Gorre R, Keatley S, Green A, Turner P, Kallingappa PK, Verma V, Oback B. PLoS One. 2011;6(9):e24501.
Induction of pluripotent stem cells from fetal and adult cynomolgus monkey - Induction of pluripotent stem cells from fetal and adult cynomolgus monkey
Okahara-Narita J, Umeda R, Nakamura S, Mori T, Noce T, Torii R. Primates. 2012 Apr;53(2):205-13.
Stress resistant human embryonic stem cells as a potential source for the - Stress resistant human embryonic stem cells as a potential source for the
Mousa SA, Sudha T, Dyskin E, Dier U, Gallati C, Hanko C, Chittur SV, Rebbaa A. Cancer Lett. 2010 Mar 28;289(2):208-16.
Induction of pluripotent stem cells from human third molar mesenchymal stromal - Induction of pluripotent stem cells from human third molar mesenchymal stromal
Oda Y, Yoshimura Y, Ohnishi H, Tadokoro M, Katsube Y, Sasao M, Kubo Y, Hattori K, Saito S, Horimoto K, Yuba S, Ohgushi H. J Biol Chem. 2010 Sep 17;285(38):29270-8.
Enhanced generation of hematopoietic cells from human hepatocarcinoma - Enhanced generation of hematopoietic cells from human hepatocarcinoma
Lu M, Kardel MD, O'Connor MD, Eaves CJ. Exp Hematol. 2009 Aug;37(8):924-36.
Expansion and differentiation of human embryonic stem cells to endoderm progeny - Expansion and differentiation of human embryonic stem cells to endoderm progeny
Lock LT, Tzanakakis ES. Tissue Eng Part A. 2009 Aug;15(8):2051-63.
Characterization of embryonic stem cell lines derived from New Zealand white - Characterization of embryonic stem cell lines derived from New Zealand white
Intawicha P, Ou YW, Lo NW, Zhang SC, Chen YZ, Lin TA, Su HL, Guu HF, Chen MJ, Lee KH, Chiu YT, Ju JC. Cloning Stem Cells. 2009 Mar;11(1):27-38.