Anti-Transglutaminase 2 antibody
| Name | Anti-Transglutaminase 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab421 |
| Prices | $391.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Guinea Pig, Human |
| Antigen | Full length native protein (purified) (Guinea Pig) |
| Description | Rabbit Polyclonal |
| Gene | TGM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This was submitted as part of a review by Dr O Fromigue
ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421. Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
Product References
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Activation and inhibition of transglutaminase 2 in mice. - Activation and inhibition of transglutaminase 2 in mice.
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