Anti-Transglutaminase 2 antibody
| Name | Anti-Transglutaminase 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab71099 |
| Prices | $384.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide from center region of human TGM2 conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | TGM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Transglutaminase 2 antibody (ab71099)Lane 1 : HUVEC lysateLane 2 : K562 cell lysateLysates/proteins at 35 µg per lane.
Immunofluorescence image of U251 cells labelling Transglutaminase 2 with ab71099. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab71099 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Transglutaminase 2 immunoreactivity is localised to the cytoplasm.
Anti-Transglutaminase 2 antibody (ab71099) at 1/60 dilution + K562 cell lysate at 35 µg
Formalin-fixed and paraffin-embedded human lung carcinoma tissue labelled with ab71099 at 1/10 diution followed by peroxidase conjugation to the secondary antibody and DAB staining.