All lanes : Anti-Translin antibody (ab71775) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate with blocking peptide at 5 µgLysates/proteins at 5 µg per lane.
Immunohistochemical staining of paraffin-embedded human brain tissue using translin antibody (ab71775) at 1/100 dilution. Staining performed in absence (left) and presence (right) of blocking peptide.
ICC/IF image of ab71775 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71775, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.