Western blot analysis of immunoprecipitation pellet from Human testis lysate immunoprecipitated using ab181862 at 1/60 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling TRAX with ab181862 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Anti-TRAX antibody [EPR13929(B)] (ab181862) at 1/5000 dilution + Human testis lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
All lanes : Anti-TRAX antibody [EPR13929(B)] (ab181862) at 1/20000 dilutionLane 1 : Mouse testis lysateLane 2 : Rat testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution