All lanes : Anti-TRIM16 antibody (ab72131) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells at 50 µgLane 2 : Whole cell lysate from Hela cells at 15 µgLane 3 : Whole cell lysate from Hela cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µgLane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Immunoprecipitation/ Western Blot of TRIM16. Lane 1: ab72131 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72131 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection:Chemiluminescence with an exposure time of 3 seconds.