Anti-TRIM65 antibody (ab170424) at 1/100 dilution + NCI-H460 cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling TRIM65 with ab170424 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells labeling TRIM65 with ab170424 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescent analysis of NCI-H460 cells labeling TRIM65 with ab170424 at a 1/10 dilution, followed by secondary antibody labeling with Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).