Anti-TRIM72 antibody
| Name | Anti-TRIM72 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab118651 |
| Prices | $376.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Rabbit, Horse, Bovine, Dog, Human, Pig, Chimpanzee, Monkey, Ape, Hamster, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TRIM72 |
| Description | Rabbit Polyclonal |
| Gene | TRIM72 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TRIM72 antibody (ab118651) at 1 µg/mlLane 1 : Heart (Mouse) Tissue LysateLane 2 : Heart (Rat) Tissue LysateLane 3 : Skeletal Muscle (Mouse) Tissue LysateLane 4 : Skeletal Muscle (Rat) Tissue LysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab118651 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab118651, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.