All lanes : Anti-Triosephosphate isomerase antibody - N-terminal (ab135532) at 1/50 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Y79 cell line lysateLane 3 : CEM cell line lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma labelling Triosephosphate isomerase with ab135532 at 1/50 dilution, followed by a peroxidase-conjugated secondary antibody and DAB staining.
Flow cytometry analysis of CEM cells labelling Triosephosphate isomerase with ab135532 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-Triosephosphate isomerase antibody - N-terminal (ab135532) at 1/50 dilutionLane 1 : Non-transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the Triosephosphate isomerase geneLysates/proteins at 2 µg per lane.